investigator: Flockhart Arrest of the normal flow of bile. An arrhythmogenic death in aortic stenosis, coronary disease, mesothelioma of the AV node, or single coronary artery. investigator: Roden Inflammation of a vessel, angiitis. Measurement of the rate of penetration of a substance through a barrier. Microsomes in liver cells. investigator: Ratain, Flockhart, McLeod investigator: Roden, Flockhart An recording of the electrical activity of the heart contraction. Assessments of the mental status of a subject. The study of the rate at which a substrate binds to or acts at a receptor. investigator: Ratain, Weinshilboum, Giacomini, Flockhart, McLeod The study of the rate at which an enzyme or transporter functions. T50 (temperature at which 50% of the activity is obtained for an enzyme). investigator: Weinshilboum A method to measure the protein half life in vitro, using radiolabeled proteins that are generated in the RRL, and radioactivity is measured on a gel which represents the remaining protein can be compared among each time points, from which half-life can be calculated. Assay using a radioactively labeled DNA probe to determine whether any proteins bind to the DNA sequence of interest. Non-radioactive probes are used to determine whether protein binding is specific to the sequences or not. Methods used to determine the phenotype of an organism, including assays, clinical measures, and observations. Abrupt loss of consciousness, often due to an abnormal rhythm. investigator: Roden Phenotyping methods using cytosolic preparations of tissues (i.e. liver), used in genotype to phenotype (or phenotype to genotype) studies. investigator: Weinshilboum A vascular reaction involving the deep dermis or subcutaneous or submucal tissues, representing localized oedema caused by dilatation and increased permeability of the capillaries and characterized by development of giant wheals. Protein/DNA binding reaction to determine whether/which transcription factors might bind to the DNA sequence of interest. Also used to determine if a variant nucleotide disrupts protein binding. Emission of blood from anywhere in the body. Abnormal clotting of blood in a blood vessel, which often results in occlusion of the vessel. Damage, injury, or symptoms related to neural tissues, or abnormal neurological symptoms, often because of drug side-effects. Evaluation procedures that focus on both the outcome or status (outcome assessment) of the patient at the end of an episode of care - presence of symptoms, level of activity, and mortality; and the process (process assessment) - what is done for the patient diagnostically and therapeutically. A 17-point screening instrument designed to measure the severity of depression. investigator: Licinio, Giacomini investigator: Ratain, Weinshilboum An antibody-based method for detecting specific proteins. investigator: Giacomini Refers to expression of a protein of interest in a yeast expression system. The quantity or amount of drug in the blood plasma. A method for assaying quantitative gene expression. investigator: Ratain, Flockhart, McLeod, Weiss, Weinshilboum The blood pressure, measured daily. investigator: O'Connor Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. investigator: Ratain investigator: Ratain, McLeod The rate of recurrence of a disease. Microarrays containing antibodies used to assay proteins. A cancer different from a cancer originally diagnosed in a patient. investigator: Ratain, Relling Whole-organism methods used to determine the phenotype of an organism. Protein half-life is a measure of the protein turn over rate of a protein in the cell. investigator: Roden, Flockhart Measures of how well the lungs take in and exhale air and how efficiently they transfer oxygen into the blood. investigator: Giacomini, Flockhart, Weinshilboum The dissociation constant of an inhibitor; in enzyme kinetics. Immunoprecipitation is a way to look at the protein complex or protein-protein interactions. A method to measure the protein half-life in the cell system, using cells that are incubated with radiolabeled amino acid, and immunoprecipitation (IP) can be performed using antibody against the protein interested. IP lysates are then run on the gel and radioactivity of the remaining proteins can be measured and compared among each time points. Pain in a muscle or muscles. investigator: Krauss The formation and accumulation of an abnormally large number of eosinophils in the blood. investigator: Krauss Measurements related to the renin/angiotensin system, such as Angiotensinogen, Angiotensin I/II, plasma renin, etc. Microarray experiments containing oligonucleotides. investigator: Ratain, Relling, Flockhart, McLeod, Weiss The quantity or amount of homocysteine in the in plasma, CSF, or other fluids. The frequency of patient visits the the emergency room. investigator: Weiss investigator: McLeod Emission of blood emanating from the gastrointestinal tract. Measurements applied to the whole individual or to samples from that individual. A specialized imaging technique using short lived radioactive substances. Measurements of a physical nature on an individual. A technique used to determine the mass, composition, and identity of proteins. A molecular tag for measuring the location or expression of a protein. Microarray experiments in which spotted arrays are used. investigator: Flockhart, Weiss Methods used to determine the genotype of an organism, including sequencing assays and polymorphism detection methods. The frequency of occurrences of hospitalizations for acute asthma attacks. investigator: Weiss A decrease in the number of platelets in the blood. investigator: McLeod The individuals’ perceptions of their position in life, in the context of the cultural and value systems in which they live and in relation to their goals, expectations, standards and concerns. Measurements applied to samples of blood or tissue from an individual. A technique for mRNA detection and quantitation. investigator: Ratain, Flockhart, McLeod, Weiss, Weinshilboum Measurements of the catalytic action or potency of enzymes, such as measured by enzyme kinetics. A form of syncope (fainting) that occurs as a part of a normal physiologic response to stress (often emotional stress). The individual becomes lightheaded, nauseated, flushed, feels warm and then may lose consciousness for several seconds. investigator: Krauss A test measuring the bronchial contractility of the airways after administration of Methacholine. investigator: Weiss These are microarrays used to detect proteins, monitor their expression levels, and investigate protein interactions and functions. Assay where antibody to a protein of interest is added to see whether the protein of interest is participating in the protein binding complex. investigator: Weiss, Weinshilboum This is a general category of experiments related to assessment of the activity of a promoter. investigator: Weiss, McLeod The rate of cure or response to a disease. investigator: Roden An abnormal rhythm associated with an irregular heart rate. investigator: Giacomini Kinetics of transport related to a single channel. Includes total cholesterol, HDL-cholesterol (often called good cholesterol), LDL-cholesterol (often called bad cholesterol), and triglycerides, and associated computed parameters. investigator: Krauss, Flockhart Kinetic measures related to the uptake of a substance. investigator: Giacomini, Weinshilboum These are measurements related to the transformation of substances in the body. E.g., viremia, fungemia, etc. Refers to expression of a protein of interest in an insect cell expression system. restriction fragment length polymorphism A convenient method for detecting SNPs is restriction fragment length polymorphism (SNP-RFLP). If one allele contains a recognition site for a restriction enzyme while the other does not, digestion of the two alleles will give rise to fragments of different length. Currently, the study of existing SNPs are most easily studied using microarrays. Microarrays allow the simultaneous testing of over a thousand separate SNPs and are quickly screened by computer. Several other ways to detect SNP's are mentioned in an article published in October 2005 in Nature by the International HapMap Consortium. [Ref: wikipedia] investigator: Krauss Abnormally low blood pressure, seen in shock but not necessarily indicative of it. Scarring or thickening of tissues deep in the lung. investigator: McLeod A test measuring the clearance of Erythromycin, and is an indicator of the metabolism of other drugs as well. investigator: O'Connor, Krauss, Roden Measurement is divided into systolic (pressure during contraction of the heart) and diastolic (pressure during relaxation phase). A nuclear medicine test that estimates the mineralization of bone in a subject. investigator: Flockhart A decrease in the rate or depth of respiration respiratory effort and decrease in level of alertness. investigator: Weiss investigator: McLeod Tissue arrays are glass slides containing spotted tissue samples, used for protein expression studies, antibody screening, tissue-type specificity studies and animal model analyses. Refers to expression of a protein of interest in the Xenopus oocytes. investigator: Giacomini The length of time a subject lives without having one or more complications of a disease. The frequency of occurrences of medical events or conditions subsequent to heart attacks. The science and study of the factors which determine the amount of chemical agents at their sites of biological effect at various times after the application of an agent or drug to biological systems. Pharmacokinetics includes study of drug absorption and distribution (biotranslocation), study of the chemical alterations a drug may undergo in the body, (biotransformation), and study of the means by which drugs are stored in the body and eliminated from it. investigator: Ratain, Relling, Flockhart, McLeod, Giacomini, Roden An unbiased method can help us detect any associated proteins, using LC-MS/MS to separate the cell mixtures or lysates and run through MS/MS to detect all the possible associated proteins. Forced Expiratory Volume in 1 second. investigator: Weiss A panel of detailed lipid, apolipoprotein, and lipoprotein subspecies measurements used for research purposes. investigator: Krauss Injury and/or death of liver cells due to a drug or toxin. investigator: Ratain, Relling, Flockhart, Krauss investigator: Roden, Giacomini Measurements of the electrical phenomena associated with cardiac or other physiology. Physiological variations that occur when a subject partakes in a defined exercise activity. investigator: Roden investigator: Weiss Forced Vital Capacity. This is the total amount of air a subject can blow out forcefully. Use of biological tools such as immunoprecipitation or proteomics to look at which proteins might interact with the proteins of interest. investigator: Weinshilboum A cancer that develops in a patient. All side effects directly attributable to cancer treatment. investigator: Ratain, Relling, Flockhart investigator: Ratain Methods that assess the length of the mRNA transcript. investigator: Roden, Flockhart Time from electrocardiogram Q wave to the end of the T wave corresponding to electrical systole. Measurements of the quantity or amount of protein in a cell, space, or assay. The overall catalytic rate of an enzyme; symbol for turnover number; Vmax divided by the total enzyme concentration. investigator: Ratain, Giacomini investigator: Flockhart, Roden Measures the speed of blood coagulation at different steps of the coagulation pathway. These are functional measures of lymphocyte responses. investigator: Ratain An repetitive, uncontrollable excitation of neural tissue, usually resulting in violent muscular contractions. E.g., CD4 cell counts. All tissue-based methods used to determine the phenotype of an organism. An organism living to or beyond a specified time. A time period during which an organism remains alive. investigator: Ratain, Relling, McLeod, Weinshilboum investigator: Relling Measurements on proteins in blood serum. Microsomes in kidney cells. The introduction of a protein into a cell that does not normally express that protein. investigator: Weinshilboum Undesirable actions of drugs on subject, of all severity. investigator: Ratain, Giacomini, Flockhart, Weinshilboum The maximum initial velocity of an enzyme catalyzed reaction. I.e. at saturating substrate levels. Assays or measurements of molecular targets or related to the kinetics of molecular components. investigator: Weinshilboum A kinetic parameter used to characterize an enzyme, defined as the concentration of substrate that permits half maximal rate of reaction. investigator: Ratain, Giacomini, Flockhart, Weinshilboum These are laboratory tests related to lung function. investigator: Krauss A study recording the blood pressure at intervals during a 24-hour period. The rate of elimination of a drug, metabolite, or other substance from the body or organ of interest. The sensation of sudden flushing and sweating. investigator: Flockhart Multiple proteins interact with each other to form a complex. It can be a physical interaction, which can have no biological effects, or biological interaction which can interact indirectly. Proteins usually function as complexes rather than as a single one. investigator: Ratain, Flockhart, Weiss, Weinshilboum All methods that assay gene expression. Refers to expression of a protein of interest in a mammalian cell expression system. investigator: Flockhart, Roden, Weinshilboum